بررسی مقاومت آنتی بیوتیکی در سالمونلا اینفنتیس جدا شده از ماکیان شهرستان اراک

نوع مقاله: مقاله پژوهشی (اصیل)

نویسنده

موسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج

چکیده

سالمونلوز بیماری مشترک بین انسان، دام و طیور میباشد که بعنوان بیماری منتقله از مواد غذایی نیز مطرح می باشد. یکی از سروتیپ های شایع این باکتری ، سالمونلا اینفنتیس می باشد. هدف از انجام این تحقیق بررسی مقاومت آنتی بیوتیکی در 70سالمونلا اینفنتیس جدا شده از ماکیان طی سال های 1390-1389 در شهرستان اراک می باشد. در بررسی ژنوتیپی جهت تایید جنس، تمام جدایه ها باند 5/1 کیلو بازی را از خود نشان دادند. نتایج آنتی بیوگرام بر اساس استاندارد جهانی CLSI و با استفاده از روش دیسک دیفیوژن(Kirby-Bauer) نشان داد که 100% جدایه ها به آنتی بیوتیک های نالیدیکسیک­اسید(NA) و نیتروفورانتوئین(FM)  مقاومت نشان دادند.  از بین 70 جدایه ، 2(9/2%) مورد به 11 آنتی بیوتیک، 10 (3/14%) مورد به 10 آنتی بیوتیک، 8 (4/11%) مورد به 9 آنتی بیوتیک، 6 (6/8%) مورد به 8 آنتی بیوتیک، 22(4/31%) مورد به 7 آنتی بیوتیک، 12 (1/17%) مورد به 6 آنتی بیوتیک و 8 (4/11%) مورد به 5 آنتی بیوتیک مقاوم بودند. همچنین 29 الگوی مقاومت آنتی بیوتیکی بدست آمد.

کلیدواژه‌ها

موضوعات


1. Anonymous (2010a). The community summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in the European Union in 2008. The EFSA Journal, 1496.  

2. Anonymous (2010c). ECDC surveillance report; Annual epidemiological report on communicable diseases in Europe. Stockholm;2101.www.ecdc.europa.eu/en/publications/surveillance_reports/ /Pages/index.aspx

3. Ashtiani, M.T., Monajemzadeh, M., Kashi, L.(2009). Trends in antimicrobial resistance of fecal Shigella and Salmonella isolates in Tehran, Iran. Indian Journal of Pathology & Microbiology 52:52–5.

 4. Asgharpour, F., Rajabnia, R., Ferdosi Shahandashti, E., Marashi, M.A., Khalilian, M., Moulana, Z.(2014). Investigation of class I integron in Salmonella infantis and its association with drug resistance.  Jundishapur Journal of Microbiology 7: e10019.

 5. Biedenbach, D.J., Toleman, M.,Walsh, T.R., Jones, R.N. (2006): Analysis of Salmonella spp. with resistance to extended-spectrum cephalosporins and fluoroquinolones isolated in North America and Latin America: report from the SENTRY Antimicrobial Surveillance Program (1997–2004). Diagnosis Microbiology Infectious Disease 54: 13–21.

 6. Beyene, G., Nair, S., Asrat, D., Mengistu, Y., Engers, H., Wain, J.(2011). Multidrug resistant Salmonella concord is a major cause of salmonellosis in children in Ethiopia .  Journal of Infectious in  Developing Countries 5:023-033.

 7. Brands, D.A., Inman, A.E., Gerba, C.P., Maré, C.J., Billington, S.J., Saif, L.A., Levine, J.F., Joens, L.A. (2005). Prevalence of Salmonella spp. in oysters in the United States. Applied & Environmental Microbiology 71: 893-897.

 8. Chashni, S.H.E., Hassanzadeh, M., Fard, M.H.B., Mirzaie, S.(2009). Characteriza­tion of the Salmonella isolates from backyard chickens in north of Iran, by serotyping, multiplex PCR and antibiotic resistance analysis. Archive of Razi Insitutet. 64:77–83.

9. Clinical Laboratory Standards Institute (2012). Performance standards for antimicrobial susceptibility testing; 21nd informational supplement. Clinical Laboratory Standards Institute, Wayne, Pa.

 10. Dahshan, H., Chuma, T., Shahada, F., Akiba, M., Fujimoto, H., Akasaka, K., Kamimura, Y., Okamoto, K. (2010). Characterization of antibiotic resistance and the emer­gence of AmpC-producing Salmonella Infantis from pigs. Journal of Veterinary Medical Science 72:1437–42.

 11. Dauga,C., Zabrovskaia,A., Grimont,P.(1998). Restriction fragment length polymorphism analysis of some flagellin genes of Salmonella enteric. Journal of Clinical Microbiology 36: 2835-2843.

 

12. Fallah, S.H., Asgharpour, F., Naderian, Z., Moulana, Z.(2013). Isolation and determination of antibiotic resistance patterns in non-typhoid salmonella spp isolated from chicken.  International Journal of Enterpathogen 01:17-21

 13. Galanis, E., Lo Fo Wong, D.M., Patrick, M.E., Binsztein, N., Cieslik, A., Chalermchikit, T., Aidara-Kane, A., Ellis, A., Angulo, F.J., Wegener, H.C. (2006). Web-based surveillance and global Salmonella distribution, 2000–2002. Emerging Infectious Disease 12: 381-388.

 14. Guerrant, R.L., Van Gilder, T., Steiner, T.S., Thielman, N.M., Slutsker, L., Tauxe, R.V., Hennessy, T., Griffin, P.M., DuPont, H., Sack, R.B., Tarr, P., Neill, M., Nachamkin, I., Reller, L.B., Osterholm, M.T., Bennish M.L., Pickering L.K.  (2001): Practice guidelines for the management of infectious diarrhea. Clinical & Infectious Disease 32: 331–351.

15. Hamidian, M., Tajbakhsh, M., Walther-Rasmussen, J., Zali, M.R.(2009). Emergence of extended-spectrum beta-lactamases in clinical isolates of Salmonella enterica in Tehran, Iran. Japanese Journal of Infectious Disease 62:368–71.

 16. Hendriksen, R.S.(2013). Molecular clonality and antimicrobial resistance in Salmonella enterica serovars Enteritidis and Infantis from broilers in three Northern regions of Iran. BMC Veterinary Research 9:66.

 17. Lee, J.Y., Kim, J.A., Jeong, H.S., Shin, J.H., Chang, C.L., Jeong, J., Cho, J.H, Mi-Na Kim, M.N., Kim, S., Young Ree Kim, Y.R., Lee, C.H., Lee, K., Lee, M.A., Lee, W.G., Shin, J.H., Lee, J.N.(2013) Serotyping and antimicrobial susceptibility of Salmonella spp.:Nationwide multicenter study in Korea. Japanese Journal of Infectious Disease 66: 284-289.

 18. Mihaiu, L., Lapusan, A., Tanasuica, R., Sobolu, R., Mihaiu, R., Oniga, O., Mihaiu, M.(2014).  First study of Salmonella in meat in Romania.  Journal of Infection in Developing Countries 8:050-058.

 19. Morshed, R., Peighambari, S.M.(2010). Drug resistance, plasmid profile and random amplified polymorphic DNA analysis of Iranian iso­lates of Salmonella enteritidis. New Microbiologica 33:47–56.

 

20. Naghoni, A., Ranjbar, R., Tabaraie, B., Farshad, S., Owlia, P., Safiri, Z., Mammina, C. (2010). High prevalence of integron-mediated resistance in clinical isolates of Salmonella enterica. Japanese Journal of Infectious Disease63:417–21.

 21. Nógrády, N., Toth, A., , Kostyak, A.,  Paszti, J., Nagy, B.(2007). Emergence of multidrug resistant clones of Salmonella Infantis in broiler chickens and humans in Hungary. Journal of Antimicrobial Chemotherapy 60, 645–648.

 22. Nógrády, N., Király, M., Davies, R., Nagy, B.(2012). Multidrug resistant clones of Salmonella Infantis of broiler origin in Europe. International Journal of Food Microbiology 157:108-12.

 

23. Rahmani, M., Peighambari, S.M., Svendsen, C.A., Cavaco, L.M., Agerso, Y., Hendriksen, R.S. (2013). Molecular clonality and antimicrobial resistance in Salmonella enterica serovars Enteritidis and Infantis from broilers in three Northern regions of Iran. BMC Veterinary Research 9:66. 

 24. Swartz, M.N. (2002). Human diseases caused by food-borne pathogens of animal origin. Clinical Infectious Disease 34: S111-S122.

   25.  Tajbakhsh, M., Yaghobi Avini, M., Khajeh, J.A., Alebouyeh, M., Nazemalhoseini Mojarad, E., Zali, M.R.(2012).Increased-resistance phenotype resulted from elevated β-lactamase enzyme activity in Salmonella clinical isolates. Journal of Isfahan Medical School 30:1-11.

 26.Vandeplas, S., Dubois Dauphin, R., Beckers, Y., Thonart, P., Thevis, A. (2010). Salmonella in chicken: Current and developing strategies to reduce contamination at farm level. Journal of Food Protection 73, 774-785.

 27. Wagenaar, J.A., Hendriksen, R.S., Carrique-Mas, J.(2013). Practical considerations of surveillance of Salmonella serovars other than Enteritidis and Typhimurium. Scientific and Technical Review of the Office International des Epizooties 32:509-519.

 28. WHO. (2001). (ITS Global Strategy for Containment of Antimicrobial Resistance) WHO, Geneva. WHO/CDC/CSR/DRS/2001.2.

 29. Yan, H., Li, L., Alam, M.J., Shinoda, S., Miyoshi, S., Shi, L.(2010). Prevalence and antimicrobial resistance of Salmonella in retail foods in north­ern China. International Journal of Food Microbiology 143:230–4.

 30. Zhao, S., White, D.G., Friedman, S.L., Glenn, A., Blickenstaff, K., Ayers, S.L., Abbott, J.W., Hall-Robinson, E., McDermott, P.F. (2008). Antimicrobial resistance in Salmonella enterica serovar Heidelberg isolates from retail meats, including poultry, from 2002 to 2006. Applied & Environmental Microbiology 74: 6656-6662.